Tottenham have agreed a five-year deal to host a Saracens rugby union fixture once a season.
The Tottenham Hotspur stadium will stage Saracens' 'Big Game' Gallagher Premiership fixture from 2020 to 2025.
Rugby union joins football and NFL as sports to be hosted at the new ground in north London.
The first match of the arrangement will be held in 2020 and takes the dominant force in English domestic rugby away from London Stadium and Wembley, the previous venues used for a clash that is usually played against capital rivals Harlequins.
Tottenham chairman Daniel Levy said: "This agreement with Saracens further reinforces our aspiration to deliver a world-class multi-use venue in our home of Tottenham.
"This is an exciting partnership and, alongside Premier League and NFL matches, it is one that continues to underline our desire to create a sports and entertainment destination that we believe can transform this area of London."
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